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KMID : 0829220090330010019
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Korean Journal of Oral and Maxillofacial Pathology
2009 Volume.33 No. 1 p.19 ~ p.26
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Methanol Extract of Leaves from Cudrania tricuspidata Effects in HT-29 Colorectal Adenocarcinoma
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Kim Mi-Sook
Kim In-Ae
Ko Young-Jong
Jeong JIn An
Kim JI-Eun
Song Byung-Jun
Lim Won-Bong
Lim Hoi-Soon
Park Ji-Il
Kim Seo-Yune
Choi Hong-Ran
Kim Ok-Joon
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Abstract
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The phytochemicals of many plants suggest their potential use as dietary supplements in cancer chemoprevention and chemotherapy. In the present study, antitumor activity of Cudrania tricuspidata, a plant native to East Asia, was investigated. Cell growth inhibition of the extract on HT-29 colorectal adenocarcinoma using MTT colorimetric assay was determined. Apoptosis on HT-29 cells was performed by DNA fragmentation analysis. PGE2 release was measured by enzyme immunoassay, because PGE2 is a key protumorigenic prostanoid in many human cancers. For the ROS scavenging activity, ROS level was detected by laser scanning confocal microscope. It was found that methanol extract of leaves inhibits cell viability by inducing apoptosis as evidenced by DNA fragmentation. Stem bark decreases synthesis of PGE2, inflammatory mediator. Fruits exhibited pronounced ROS scavenging activity. Taken together, these results suggest that Cudrania tricuspidata exerts growth inhibition and anti-oxidation on HT-29 cells through apoptosis, ROS scavenging respectively that it may have anti-cancer properties.
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KEYWORD
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Cudrania tricuspidata, Anti-inflammation, Anti-oxidation
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